Liver orcein stain and DHBV DNA.
نویسندگان
چکیده
منابع مشابه
Orcein as a mucin stain for gastrointestinal tissue sections.
Although orcein has been in use for a long time as a stain for elastic fibres, it is only recently that it has been rediscovered for the demonstration of a variety of other substances. These include hepatitis B surface antigen (HBsAg),' 2 copper-associated protein,34 and more recently, intestinal sulphated mucins.35 Sipponen5 studied the normal distribution of orceinpositive mucin in the gastro...
متن کاملOrcein staining of hepatitis B antigen in paraffin sections of liver biopsies.
Liver biopsies from 97 hepatitis B antigen (HBsAG)-positive patients were stained by a modified orcein method described by Shikata et al (1974) in order to detect the antigen in liver tissue. The results were consitently negative in acute hepatitis, but positive in nearly two-thirds of biopsies from 53 patients with chronic liver disease. The distribution of positive staining was frequently irr...
متن کاملUse of orcein in detecting hepatitis B antigen in paraffin sections of liver.
This study has shown that different supplies/batches of orcein perform differently and may fail. The "natural" forms generally performed better although the most informative results were obtained with a "synthetic" product. Orcein dye solutions can be used soon after preparation and for up to 7 days without the need for differentiation. After 10 days or so the staining properties become much le...
متن کاملDuck hepatitis B virus DNA in liver and serum of Chinese ducks: integration of viral DNA in a hepatocellular carcinoma.
The presence of duck hepatitis B virus (DHBV) DNA in liver and serum and its state (integrated vs. free) were studied in 23 ducks from Chi-tung county in China by spot hybridization and Southern blot hybridization, respectively. In 16 of 23 (70%), DHBV DNA was detected in serum and/or in liver tissue. These infected ducks showed a variety of pathological changes including advanced chronic disea...
متن کاملDuck hepatitis B virus replicates in the yolk sac of developing embryos.
Duck hepatitis B virus (DHBV) is the only member of the hepadnavirus family in which nearly 100% vertical transmission from carrier mother to embryo has been reported. Large quantities of maternally transmitted virus particles are present in the yolk prior to incubation of the eggs, and replicative forms of DHBV DNA are detectable in the liver at 6 days of incubation. Since the yolk sac is simi...
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ژورنال
عنوان ژورنال: Kanzo
سال: 1985
ISSN: 0451-4203,1881-3593
DOI: 10.2957/kanzo.26.856